rnascope® multiplex tsa buffer  (Advanced Cell Diagnostics Inc)

Journal: Cell Reports Medicine

Article Title: The μ-opioid receptor differentiates two distinct human nociceptive populations relevant to clinical pain

doi: 10.1016/j.xcrm.2024.101788

Figure Lengend Snippet:

Article Snippet: RNAscope® Multiplex TSA Buffer , Advanced Cell Diagnostics , CAT#322810.

Techniques: RNAscope, Multiplex Assay, Software, Microscopy

Journal: Cell reports

Article Title: Melanocortin-3 receptor expression in AgRP neurons is required for normal activation of the neurons in response to energy deficiency

doi: 10.1016/j.celrep.2023.113188

Figure Lengend Snippet: (A and B) Validation of Cre-dependent MC3R-FLAG viral expression in AgRP Cre:L10-EGFP mice by immunostaining against FLAG, EGFP, and AgRP. Scale bar, 50 μm for the ARC and 100 μm for the PVN. (C and D) RNAscope analysis of Mc3r and Agrp mRNA expression, and quantifications of the percentage of AgRP-positive cells coexpressing MC3R in the ARC of AgRP-Cre and AgRP-specific MC3R KO male mice. Scale bar, 100 μm (n = 3 mice for each group). (E‒G) Body weight and body composition of AgRP-Cre and AgRP-specific MC3R KO male mice (n = 8 mice for AgRP-Cre and n = 12 for AgRP-specific MC3R KO groups). (H) 2-h food intake of AgRP-Cre and AgRP-specific MC3R KO male mice following a 24-h fast (n = 6 mice for AgRP-Cre and n = 8 for AgRP-specific MC3R KO groups). (I and J) Representative images of cFos immunostaining, and quantifications of cFos-positive cell number in the ARC of 24-h-fasted AgRP-Cre and AgRP-specific MC3R KO male mice. Scale bar, 100 μm (n = 3 mice for AgRP-Cre and n = 4 for AgRP-specific MC3R KO groups). (K) 4-h food intake of AgRP-Cre and AgRP-specific MC3R KO male mice under RT (22°C) or cold exposure (4°C) (n = 9 mice for AgRP-Cre and n = 8 for AgRP-specific MC3R KO groups). (L and M) Representative images of cFos immunostaining, and quantifications of cFos-positive cell number in the ARC of cold-treated AgRP-Cre and AgRP-specific MC3R KO male mice. Scale bar, 100 μm (n = 4 mice for AgRP-Cre and n = 5 for AgRP-specific MC3R KO groups). Data are plotted as mean, and all error bars represent the SEM. ns, non-significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 in unpaired Student’s t test and two-way ANOVA with Sidak’s post hoc test.

Article Snippet: RNAscope ® Multiplex TSA Buffer , Advanced Cell Diagnostics , Cat# 322809.

Techniques: Biomarker Discovery, Expressing, Immunostaining, RNAscope

Journal: Cell reports

Article Title: Melanocortin-3 receptor expression in AgRP neurons is required for normal activation of the neurons in response to energy deficiency

doi: 10.1016/j.celrep.2023.113188

Figure Lengend Snippet: (A) 1-h food intake of AgRP-Cre and AgRP-specific MC3R KO male mice given saline or ghrelin injection (1.6 mg/kg, i.p.) (n = 7–8 mice for all groups). (B and C) Representative images of cFos immunostaining, and quantifications of cFos-positive cell number in the ARC of AgRP-Cre and AgRP-specific MC3R KO male mice in response to saline or ghrelin injection. Scale bar, 100 μm (n = 4–5 mice for each group). (D) Schematic showing the time course of ghrelin injection and food presence in fiber photometry experiment (E‒H) Traces and quantifications of averaged dF/F0 (%) GCaMP6s signal in AgRP neurons in WT and MC3R KO male mice in response to ghrelin and food cue (n = 4–5 mice for all groups). (I) qPCR analysis of hypothalamic GHSR mRNA levels in fed versus 24-h-fasted WT and MC3R KO male mice (n = 3–5 mice for all groups). (J‒L) RNAscope analysis of Agrp and Ghsr mRNA expression, and quantifications of the number of Ghsr transcripts in each AgRP neuron and the percentage of AgRP neurons coexpressing Ghsr in the ARC of fed versus 24-h-fasted AgRP-Cre and AgRP-specific MC3R KO male mice. Scale bar, 100 μm (n = 3–4 mice for all groups). Data are plotted as mean, and all error bars represent the SEM. ns, non-significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 in unpaired Student’s t test and two-way ANOVA with Sidak’s post hoc test.

Article Snippet: RNAscope ® Multiplex TSA Buffer , Advanced Cell Diagnostics , Cat# 322809.

Techniques: Saline, Injection, Immunostaining, RNAscope, Expressing

Journal: Cell reports

Article Title: Melanocortin-3 receptor expression in AgRP neurons is required for normal activation of the neurons in response to energy deficiency

doi: 10.1016/j.celrep.2023.113188

Figure Lengend Snippet: (A‒C) RNAscope analysis of Agrp and Gria3 mRNA expression, and quantifications of the number of Gria3 transcripts in each AgRP neuron and the percentage of AgRP neurons coexpressing Gria3 in the ARC of fed versus 24-h-fasted AgRP-Cre and AgRP-specific MC3R KO male mice. Scale bar, 50 μm (n = 3–5 mice for all groups). (D‒F) RNAscope analysis of Agrp and Ptk2b mRNA expression, and quantifications of the number of Ptk2b transcripts in each AgRP neuron and the percentage of AgRP neurons coexpressing Ptk2b in the ARC of fed versus 24-h-fasted AgRP-Cre and AgRP-specific MC3R KO male mice. Scale bar, 50 μm (n = 3–5 mice for all groups). Data are plotted as mean, and all error bars represent the SEM. ns, non-significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 in unpaired Student’s t test and two-way ANOVA with Sidak’s post hoc test.

Article Snippet: RNAscope ® Multiplex TSA Buffer , Advanced Cell Diagnostics , Cat# 322809.

Techniques: RNAscope, Expressing

Journal: Cell reports

Article Title: Melanocortin-3 receptor expression in AgRP neurons is required for normal activation of the neurons in response to energy deficiency

doi: 10.1016/j.celrep.2023.113188

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: RNAscope ® Multiplex TSA Buffer , Advanced Cell Diagnostics , Cat# 322809.

Techniques: Virus, Plasmid Preparation, Recombinant, Reverse Transcription, RNAscope, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Diagnostic Assay, Software